primary rabbit polyclonal antibody anti timp 2  (Santa Cruz Biotechnology)

Journal: International Journal of Nephrology

Article Title: Effect of the Direct Renin Inhibitor Aliskiren on Urinary Albumin Excretion in Spontaneous Type 2 Diabetic KK- A y Mouse

doi: 10.1155/2013/519130

Figure Lengend Snippet: Expression of MMP-2, MMP-9, TIMP-1, TIMP-2, fibronectin, type IV collagen, MCP-1, and (P)RR in the kidneys of each mouse using real-time PCR (A: untreated KK mice at 8 weeks of age, B: untreated KK mice at 12 weeks of age, C: untreated KK- A y mice at 8 weeks of age, D: untreated KK- A y mice at 12 weeks of age, and E: treated KK- A y mice at 12 weeks of age). The ratio of MMP-2 (a), MMP-9 (b), TIMP-1 (c), TIMP-2 (d), fibronectin (e), type IV collagen (f), MCP-1 (g), and (P)RR (h) mRNAs to GAPDH mRNA is shown in the kidneys of mice from each group. These expressions were increased significantly in group 4 compared with groups 1, 2, and 3. This increase was attenuated in group 5 (* P < 0.001 versus untreated KK- A y mice at 12 weeks of age).

Article Snippet: The primary antibodies (Abs) were as follows: goat polyclonal anti-MMP-2 Ab (R&D Systems, Inc., Minneapolis, MN, USA), goat polyclonal anti-MMP-9 Ab (R&D Systems, Inc.), rabbit polyclonal anti-TIMP-1 Ab (Abbiotec, LLC, San Diego, CA, USA), rabbit polyclonal anti-TIMP-2 Ab (Abbiotec, LLC), and rat monoclonal anti-F4/80 Ab (MAC497GA; Serotec, Oxford, UK).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: International Journal of Nephrology

Article Title: Effect of the Direct Renin Inhibitor Aliskiren on Urinary Albumin Excretion in Spontaneous Type 2 Diabetic KK- A y Mouse

doi: 10.1155/2013/519130

Figure Lengend Snippet: Immunohistochemical staining of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the renal sections of each mouse (O: untreated KK- A y mice at 12 weeks of age without primary Ab, A: untreated KK mice at 8 weeks of age, B: untreated KK mice at 12 weeks of age, C: untreated KK- A y mice at 8 weeks of age, D: untreated KK- A y mice at 12 weeks of age, and E: treated KK- A y mice at 12 weeks of age). Stainings showing the expression of MMP-2 (a), MMP-9 (b), TIMP-1 (c), and TIMP-2 (d) in the kidneys of mice from each group. These expressions were increased significantly in group 4 compared with groups 1, 2, and 3. This increase was attenuated in group 5 (* P < 0.001 versus untreated KK- A y mice at 12 weeks of age). Images were taken at 400-fold (a and d) and 200-fold (b and c) magnification. WGA indicates whole glomerular area. WIA indicates whole interstitial area.

Article Snippet: The primary antibodies (Abs) were as follows: goat polyclonal anti-MMP-2 Ab (R&D Systems, Inc., Minneapolis, MN, USA), goat polyclonal anti-MMP-9 Ab (R&D Systems, Inc.), rabbit polyclonal anti-TIMP-1 Ab (Abbiotec, LLC, San Diego, CA, USA), rabbit polyclonal anti-TIMP-2 Ab (Abbiotec, LLC), and rat monoclonal anti-F4/80 Ab (MAC497GA; Serotec, Oxford, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: BMC Neurology

Article Title: Matrix metalloproteinases and their inhibitors in human traumatic spinal cord injury

doi: 10.1186/1471-2377-7-17

Figure Lengend Snippet: Distribution of MMPs and TIMPs in the normal human spinal cord . Images were taken from control human spinal cords in transverse sections. A: Immunohistochemistry for MMP-1 revealed cytoplasmic staining in motoneurons; the nucleus was devoid of immunoreactivity. B: Staining for MMP-9 demonstrated a single intravascular monocyte (arrow) within a parenchymal blood vessel. C: The TIMP-2 antibody displayed cytoplasmic immunoreactivity in motoneurons, the nucleus being devoid of staining. D: Immunohistochemistry for TIMP-3 also shows cytoplasmic staining in motoneurons without a nuclear signal. (A-D, mag. ×260)

Article Snippet: Sections were subsequently incubated in the following primary antibodies, overnight at room temperature: mouse monoclonal anti-MMP-1 (1:4000; Chemicon), mouse monoclonal anti-MMP-2 (1:20; Calbiochem), mouse monoclonal anti-MMP-9 (1:100; R&D Systems), mouse monoclonal anti-MMP-12 (1:10; R&D Systems), mouse monoclonal anti-TIMP-1 (1:300; Chemicon), rabbit polyclonal anti-TIMP-2 (1:250; Chemicon), mouse monoclonal anti-TIMP-3 (1:100; Chemicon), rabbit polyclonal anti-glial fibrillary acidic protein (1:2500, DAKO) and mouse monoclonal anti-CD68 (1:50; DAKO).

Techniques: Immunohistochemistry, Staining

Journal: BMC Neurology

Article Title: Matrix metalloproteinases and their inhibitors in human traumatic spinal cord injury

doi: 10.1186/1471-2377-7-17

Figure Lengend Snippet: TIMP-1, -2 and -3 in the human spinal cord after traumatic injury . Images were taken from cases with different survival times after SCI in transverse sections. A: In a section from patients who died 2 days after SCI, immunohistochemistry for TIMP-1 demonstrated occasional cells (arrow) with a round to oval morphology at the lesion site. B: Immunohistochemistry for TIMP-2 showed an identical staining pattern, with single immunopositive rounded cells (arrow). C: At 4 days post injury, identifiable motoneurons demonstrated a cytoplasmic TIMP-2 immunoreactivity. D: 8 days after SCI, only single neurons (arrow) were stained for TIMP-2 and many motoneurons were unstained (arrowheads). E: 8 days after trauma, immunohistochemistry for TIMP-3 demonstrated cytoplasmic staining in most identifiable motoneurons close to the site of injury. F: In the same case, single TIMP-3 immunoreactive round cells could be seen (arrow). G: Four months after SCI, gray matter of the perilesional area showed mostly TIMP-3 immunoreactive motoneurons. H: Eight months after injury, multiple activated cells (arrows, presumably astrocytes) were immunopositive for TIMP-3 in areas directly surrounding the dense glial scar. (A-H, mag. ×260)

Article Snippet: Sections were subsequently incubated in the following primary antibodies, overnight at room temperature: mouse monoclonal anti-MMP-1 (1:4000; Chemicon), mouse monoclonal anti-MMP-2 (1:20; Calbiochem), mouse monoclonal anti-MMP-9 (1:100; R&D Systems), mouse monoclonal anti-MMP-12 (1:10; R&D Systems), mouse monoclonal anti-TIMP-1 (1:300; Chemicon), rabbit polyclonal anti-TIMP-2 (1:250; Chemicon), mouse monoclonal anti-TIMP-3 (1:100; Chemicon), rabbit polyclonal anti-glial fibrillary acidic protein (1:2500, DAKO) and mouse monoclonal anti-CD68 (1:50; DAKO).

Techniques: Immunohistochemistry, Staining

Journal: Scientific Reports

Article Title: Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling

doi: 10.1038/s41598-018-29023-3

Figure Lengend Snippet: The effects of TP on the MMP-2, MMP-9, TIMP-1 and TIMP-2 in the renal tissue of aged rats. Bar graphs show MMP-2 mRNA ( a ), MMP-9 mRNA ( b ), TIMP-1 mRNA ( c ) and TIMP-2 mRNA ( d ). ( e ) Western blot study revealed MMP-2, MMP-9, TIMP-1 and TIMP-2 expression in the renal tissue. Bar graphs illustrate the protein expression of MMP-2 ( f ), MMP-9 ( g ), TIMP-1 ( h ) and TIMP-2 ( i ). The asterisks show significant differences (* P < 0.05, ** P < 0.01).

Article Snippet: Membranes were blocked with 5% skimmed milk 1 h at room temperature, and then were probed with mouse monoclonal anti-collagen I antibody (Abcam, ab90395, 1:1000), rabbit polyclonal anti-collagen IV antibody (Abcam, ab6586, 1:1000), rabbit monoclonal anti-fibronectin antibody (Abcam, ab45688, 1:5000), rabbit polyclonal anti-MMP-2 antibody (Abcam, ab37150, 1:200), rabbit polyclonal anti-MMP-9 antibody (Abcam, ab38898, 1:1000), rabbit polyclonal anti-TIMP-1 antibody (Abcam, ab61224, 1:500), rabbit polyclonal anti-TIMP-2 antibody (Abcam, ab180630, 1:500), goat polyclonal anti-TGF-β1 antibody (Santa-Cruz, C-16, sc-31609, 1:500), mouse monoclonal anti-Smad2/3 antibody (Santa-Cruz, A-3, sc-398844, 1:200), goat polyclonal anti-p-Smad2/3 antibody (Santa-Cruz, Ser 423/425, sc-11769, 1:200) and goat polyclonal anti-Smad 4 antibody (Santa-Cruz, C-20, sc-1909, 1:1000) overnight (4 °C).

Techniques: Western Blot, Expressing